Description
TB-500 (Thymosin Beta-4 Fragment) | 5 mg
Bio Glow Wellness · Research use only
Research use only. Not for human or animal consumption. Bio Glow Wellness supplies this compound to laboratories, universities and independent researchers for in vitro and analytical work. It is not a medicine, a supplement, or a therapeutic product, and it is not sold for any personal use.
About TB-500
TB-500 is a short synthetic peptide built from the section of a larger protein called thymosin beta-4 that binds actin. Thymosin beta-4 itself is 43 residues long and occurs naturally in most human cells, where it is one of the proteins that governs how the internal scaffolding of a cell is assembled and taken apart. TB-500 reproduces only the seven residue stretch responsible for that binding, with an acetyl group on one end.
One point deserves stating plainly, because vendors routinely blur it. TB-500 and thymosin beta-4 are not the same molecule. Much of the published research described below was performed on the full 43 residue protein, not on the fragment. Where a study used the full protein, its findings belong to the full protein. The Certificate of Analysis for the batch shipped identifies the material actually supplied, and that document, not this page, is the record of what is in the vial.
TB-500 Explained Simply
Read this first. The section below is written in ordinary language so that a non-specialist can follow what this compound is and where it shows up in the scientific record. It is not medical advice, it is not a claim that the compound does anything in a person or an animal, and it is not a protocol. Bio Glow Wellness sells TB-500 for laboratory research only.
The short version
A seven residue synthetic peptide copied from the active stretch of a natural human protein involved in cell movement and structure. Supplied as a laboratory research chemical only.
What it actually is
Cells are held in shape by a scaffolding made largely of a protein called actin, and that scaffolding is constantly rebuilt whenever a cell changes shape or moves. Thymosin beta-4 is one of the proteins that regulates the supply of actin available for that rebuilding. TB-500 is the small piece of thymosin beta-4 that does the actual binding, synthesized on its own. It is far cheaper to make than the full protein and easier to handle, which is why it appears in laboratories at all.
Where it turns up in research
- Cell migration assays, where actin turnover determines how quickly cells move across a surface
- Cardiac tissue models, the area where the parent protein has the deepest published record
- Corneal and epithelial wound models in animals
- Angiogenesis work, meaning the growth of new blood vessels
- Inflammatory signalling studies in cultured cells
- Hair follicle and dermal papilla cell research
What the published work shows so far
Published work reports that thymosin beta-4 influences how fast cells migrate into an experimental wound, and describes effects on blood vessel formation and on inflammatory signalling in animal and cell models. The cardiac literature is the most developed of these. Two limits matter when reading it. Most of that work used the full protein rather than the fragment sold here, and all of it is preclinical. Controlled human trials establishing what TB-500 does in a person have not been published, and Bio Glow Wellness makes no claim on the subject.
Why researchers keep returning to it
For a laboratory studying cytoskeletal dynamics, a short peptide that binds actin is a useful and inexpensive probe. Its value as a reagent comes from being small, stable and easy to obtain in consistent quality. That is a statement about laboratory logistics. It says nothing about safety or effect in a living person.
What nobody can claim about TB-500
- It cannot be described as a treatment or a recovery aid for any injury or condition.
- It cannot be presented as equivalent to thymosin beta-4. The fragment and the full protein are different molecules with different published records.
- It cannot be described as safe or effective for humans, because the controlled trials that would support such a statement do not exist.
- Results from mice, rats or cultured cells cannot be restated as though they were human findings.
- It is not approved by the FDA for any use, and it is prohibited in sanctioned sport under the World Anti-Doping Agency code.
Where the information above comes from
Sequence and identifiers come from public chemistry references. The research summary describes the general shape of the peer-reviewed literature on thymosin beta-4 and its fragments, which is preclinical throughout. Read the primary papers rather than any vendor summary, this one included, and check whether a given study used the full protein or the fragment before drawing a conclusion from it.
Physical and Chemical Data
Reference values for TB-500 (Thymosin Beta-4 Fragment). Lot-specific results are on the Certificate of Analysis supplied with the batch.
| Molecular formula | C39H69N9O14 (acetylated seven residue fragment) |
| Molecular weight | 889.0 g/mol (fragment) |
| Chain length | 7 residues, acetylated at the N terminus |
| Sequence | Ac-Leu-Lys-Lys-Thr-Glu-Thr-Gln-OH |
| CAS number | 77591-33-4 (parent protein, thymosin beta-4) |
| Classification | Synthetic acetylated fragment corresponding to residues 17 to 23 of thymosin beta-4, the actin binding region |
| Synonyms | Thymosin Beta-4 fragment · Tβ4 17-23 · Ac-LKKTETQ |
| Appearance | White lyophilized solid |
| Reported solubility | Reported soluble in water and in bacteriostatic water at laboratory working concentrations |
Molecular Design and Stability
The design question behind this peptide is whether a binding function spread across a large protein can be reduced to the handful of residues that actually perform it. Thymosin beta-4 binds actin through a short central motif, and TB-500 is that motif isolated and capped with an acetyl group at the N terminus. Acetylation removes a free positive charge and is a standard way to slow down the enzymes that trim peptides from that end.
At seven residues the molecule is small, water soluble and stable as a freeze-dried solid. In solution it behaves like any short peptide and should be treated as short-lived. Aliquot to the working volume rather than keeping a reconstituted vial.
Laboratory Applications
Researchers working with this compound have used it to investigate:
- Scratch and transwell migration assays in cultured cells
- Actin polymerization and sequestration experiments
- Rodent models of cardiac and dermal tissue injury
- Corneal epithelial repair models
- Angiogenesis and endothelial tube formation assays
- Comparative studies of the fragment against full length thymosin beta-4
These are descriptions of published experimental work. They are not suggestions for what a buyer should do with the material, and they are not evidence of any effect outside the model in which it was observed.
What the Literature Covers
1. Actin binding, the mechanism everything else rests on
The one uncontested property is that this sequence binds monomeric actin. Everything else in the literature is an attempt to trace consequences of that binding through a cell or a tissue. Any claim about this peptide that cannot be connected back to actin handling should be read with suspicion.
2. Cardiac models
The largest and best funded body of work sits in cardiac research, where thymosin beta-4 has been studied in rodent models of heart injury and in the biology of the epicardium. This work overwhelmingly used the full protein.
3. Corneal and dermal repair
A second cluster examines surface tissue, particularly the cornea, where the protein reached clinical investigation in its own right. Findings from those programmes belong to the full protein and to the specific formulation tested, not to a research fragment bought as a powder.
4. Vascular effects
Several groups report effects on endothelial cell behaviour and vessel formation in culture and in animals. The mechanisms proposed generally run through cytoskeletal reorganization, which is consistent with the actin binding role.
5. The fragment versus the protein
This is the thread a careful reader should hold onto. Research on the seven residue fragment specifically is thin next to the literature on the full protein. Treating the two as interchangeable is the most common error made about this compound, and it is made constantly in material written to sell it.
Batch Documentation
Every shipment is tied to the paperwork for the lot it came from.
- A Certificate of Analysis is supplied for the batch shipped, where one is available for that lot.
- The COA states the analytical methods used and the results for that specific lot. It is the authoritative record for the material in hand.
- Figures on a COA describe one batch only. They are not carried over to other lots and should not be read as a standing specification for the product.
- Testing is performed by an independent third-party analytical laboratory. The laboratory is named on the certificate.
- Published certificates are collected in the Certificate of Analysis library. Our guide to reading a certificate explains what each section reports and what it does not.
Storage and Handling Guidance
General handling practice for lyophilized research peptides:
- Keep the sealed vial cool, dry and out of direct light.
- For anything beyond short-term storage, refrigerate or freeze in line with the handling standard your laboratory already follows for lyophilized material.
- Limit how often a vial is opened and closed. Each cycle exposes the powder to ambient humidity.
- Reconstituted material is generally less stable than the dry powder. Where a study calls for solution, common practice is to aliquot to the working volume rather than store the whole vial in solution.
- Let a refrigerated vial reach room temperature before opening it, so condensation does not form on the contents.
Bio Glow Wellness does not supply reconstitution volumes, concentrations, schedules or any other instruction for administration to a person or an animal. Handling decisions belong to the researcher and to the standards of the facility doing the work.
Common Questions
Can this be used by a person?
No. It is supplied for in vitro laboratory research only. It is not approved for human or veterinary use, it is not a drug or a supplement, and Bio Glow Wellness will not supply it for any other purpose.
What do researchers use TB-500 for?
It is used as a laboratory probe in work on actin dynamics and cell migration, and in animal models of tissue repair. What a given laboratory does with it depends on the study.
Do you provide dosing or reconstitution instructions?
No. Bio Glow Wellness does not publish dosing, administration or protocol guidance of any kind. Study design, handling and safety practice are the responsibility of the researcher and their institution.
Is a Certificate of Analysis included?
Where one is available for the lot shipped, yes. The COA is specific to that batch and reports the methods run and the results obtained for it. Availability varies by lot.
How is the material supplied?
As a white lyophilized powder in a sealed vial, labelled with the compound name and the vial size. The milligram figure is the quantity of peptide in the vial. It is not a potency or a dose.
Do you ship internationally?
Orders ship within the United States. Buyers outside the United States are responsible for their own import rules, and orders may be declined where the compound is restricted.
Terms of Sale and Disclaimers
- Sold for laboratory research, analytical and in vitro use only.
- Not for human consumption, not for veterinary use, and not for any form of administration to a living subject.
- Not evaluated by the Food and Drug Administration. Nothing on this page is intended to diagnose, treat, cure or prevent any disease or condition.
- No statement on this page is a representation that the compound is safe or effective for any use.
- The purchaser is responsible for meeting every federal, state and local requirement that applies to buying, holding, using and disposing of research compounds, and for confirming the compound is lawful in their jurisdiction before ordering.
- By ordering, the purchaser confirms they are a qualified researcher or are buying on behalf of a research organization.
